Welcome to RootReader2D

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This site provides an introductory guide to setup and run RootReader2D.

RootReader2D is a stand-alone Java1-based program that is designed to assist with root length measurements from digital images (Clark et al., 2013). This software allows for high-throughput analysis of total root system length as well as measurements of selected root types of interest. RootReader2D has been adapted to work with a variety of growth systems, including hydroponics, gellan gum, sand, agarose plates and paper pouch systems.


The new stand-alone version of RootReader2D (version 4.3) no longer requires Java Web Start (JAWS) or access to our website. The previous functions of RootReader2D have remained the same. Users with older versions of RootReader2D will need to download this new version.

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